Crops for Health
Dr. Heuberger’s laboratory studies biochemical diversity in fruits and vegetables, cereals, and pulse crops. The major goals of this research are to characterize the influence of genetic and environmental factors on plant metabolism, and understand how these factors affect health traits of food crops. By identifying the links between plant genetics and metabolism, it is possible to facilitate breeding for food crops with improved biochemical traits, specifically for nutrients and other phytochemicals that affect human health. Additional areas of research include identifying plant metabolites associated with sensory quality in foods, and evaluating the utility of mass spectrometry-based metabolite profiling to facilitate breeding of quality traits in crops. This research integrates techniques in the fields of metabolomics, analytical chemistry, plant breeding and genomics.
Dr. Heuberger is also a faculty consultant with the Proteomics and Metabolomics Facility at CSU. He is actively engaged in research projects that apply mass spectrometry to investigate biology and food science. This research spans many biological systems including plants, mammals, microorganisms, and plant-based and fermented foods.
Courses I Teach:
HORT 578 – Phytochemicals and Probiotics for Health
HORT 579 – Metabolomics Methods and Analysis
-H Bettenhausen, L Barr, CD Broeckling, J Chaparro, C Holbrook, D Sedin, AL Heuberger. “An influence of malt source on beer chemistry, flavor and flavor stability” (2018) Food Research International 113: 487-504.
-JM Chaparro, CD Broeckling, JE Prenni, DG Holm, AL Heuberger. “Metabolomics and ionomics of potato tuber reveals an influence of cultivar and market class on human nutrients and bioactive compounds” (2018) Frontiers in Nutrition (5) 36. DOI: 10.3389/fnut.2018.00036
-FM Robison, MF Turner, CE Jahn, HF Schwartz, JE Prenni, MA Brick, AL Heuberger. “Common bean varieties demonstrate differential physiological and metabolic responses to the pathogenic fungus Sclerotinia sclerotiorum”. Plant, Cell and Environment 41 (9) 2141-2154 DOI 10.1111/pce.13176
-F Lavergne, CD Broeckling, SD Haley, F Peairs, CE Jahn, AL Heuberger, “GC-MS metabolomics to evaluate composition of plant cuticular waxes in Triticum aestivum. (2018) International Journal of Molecular Sciences. 19 (2) 249; special issue: Metabolomics in the Plant Sciences) DOI 10.3390/ijms19020249
-AL Heuberger, CD Broeckling, D Sedin, C Holbrook, L Barr, K Kirkpatrick, JE Prenni. “Evaluation of non-volatile metabolites in beer stored at high temperature as an accelerated method to predict flavor stability.” (2016) Food Chemistry, 200, 301-307. DOI: 10.1016/j.foodchem.2016.01.022
-Heuberger, AL., Broeckling CD, Sedin D, Holbrook C, Guerdrum L, Kirkpatrick K, Prenni JE “Evaluation of non-volatile metabolites in beer stored at high temperature and utility as an accelerated method to predict flavor stability.” Food Chemistry (2016).
-Sessions-Bresnahan, DR., Heuberger AL, Schauer KL, Carnevale EM. “Effect of obesity on the preovulatory follicle and lipid fingerprint of equine oocytes.” Biology of Reproduction (2015): biolreprod-115.
-Robison FM, Heuberger AL, Ogg B, Schwartz HF, Brick MA, Prenni JE. “Proteome Characterization in Leaves of Common Bean”. (2015) Proteomes 3(3), 236-248.
-Forster GM, Heuberger AL, Broeckling CD, Bauer JE, and Ryan EP. “Cooked navy bean phytochemicals modulate the fecal and urine canine metabolome.” (2015) Current Metabolomics 3(2): 90-101.
-Sheflin AM, Borresen EC, Wdowik MJ, Rao S, Brown RJ, Heuberger AL, Broeckling CD, Weir TL, Ryan EP. (2015). “Dietary intervention with heat-stabilized rice bran modulates stool microbiota and metabolites in healthy adults.” Nutrients 7(2), 1282-1300.
-Krisher RL, Heuberger AL, Paczkowski M, Stevens J, Popsil C, Prather RS, Sturmey RG, Herrick JR, Schoolcraft WB. (2015) “Applying metabolomic analyses to the practice of embryology: physiology, development and assisted reproductive technology” Reproduction, Fertility and Development.
-Yang W, Catalonotti C, D’Adamo S, Wittkopp T, Ingram-Smith C, Mackinder L, Miller T, Heuberger AL, Peers G, Smith K, Jonikas M, Grossman A, Posewitz M. “Alternative acetate production pathways in Chlamydomonas reinhardtii during dark anoxia and the dominant role of chloroplasts in fermentative acetate production.” (2014). Plant Cell. 26(11), 4499-4518
-Weyman PD, Beeri K, Lefebvre SC, Rivera J, McCarthy JK, Heuberger AL, Peers G, Allen AE, Dupont CL (2014) “Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis.” Plant Biotechnology Journal. doi: 10.1111/pbi.12254.
-Heuberger AL, Robison FM, Lyons SM, Broeckling CD, Prenni JE (2014) “Evaluating plant immunity using mass spectrometry-based metabolomics workflows.” Frontiers in Plant Science 5:291. doi: 10.3389/fpls.2014.00291.
-Heuberger AL, Broeckling CD, Kirkpatrick K, Prenni JE (2013) “Application of non-targeted metabolite profiling to discover novel markers of quality traits in an advanced population of malting barley.” Plant Biotechnology Journal. 12 (2), 147-160.
-Abdel-Ghany S, Day I, Heuberger AL, Broeckling C, Reddy ASN (2013) “Metabolic engineering of Arabidopsis for butanetriol production using bacterial genes.” Metabolic Engineering. 20, 109-120.
-Weir T, Manter D, Sheflin A, Barnett B, Heuberger AL, Ryan EP (2013) “Fecal Microbiome and Metabolome Differences Between Colorectal Cancer Patients and Healthy Adults.” PLoS ONE 8 (8): e70803.
-Ryan EP, Heuberger AL, Broeckling C, Ollila C, Borresen E, Prenni JE. (2013) “Advances in Nutritional Metabolomics.” Current Metabolomics, 1, 109-120.
-Heuberger AL, Broeckling CD, Lewis MR, Salazar L, Bouckaert P, Prenni JE (2012) “Metabolomic profiling of beer reveals effect of temperature on non-volatile small molecules during short-term storage.” Food Chemistry. 135 (3): 1284-1289.
-Ryan EP, Heuberger AL, Weir T, Barnett B, Broeckling C, & Prenni J (2011) “Rice bran fermented with Saccharomyces boulardii generates a novel metabolite profile with bioactivity”. Journal of Agricultural and Food Chemistry. 59 (5): 1862–1870.
-Heuberger AL, Lewis MR, Chen M-H, Brick MA, Leach JE, Ryan, EP (2010) “Metabolomic and functional genomic analyses reveal varietal dfferences in bioactive compounds of cooked rice.” PLoS ONE 5(9): e12915.